Reem Aljuhani presents a poster on SULF1 structure-activity at the NIH-FDA Glycoscience Research Day
June 11th, 2025
Aljuhani, R., Benicky, J., Panigrahi, A., Mukherjee, P., Nováková, Z., Bařinka, C., Liu, J., Goldman, R. Purification and in vitro Characterization of Human Extracellular Heparan…
Dr. Panigrahi shows that Galectin-3-Binding Proptein is a specific and selective inhibitor of Sulf-2
June 11th, 2025
Galectin-3-Binding Protein Inhibits Extracellular Heparan 6-O-Endosulfatase Sulf-2. Panigrahi A, Benicky J, Aljuhani R, Mukherjee P, Nováková Z, Bařinka C, Goldman R. Mol Cell Proteomics. 2024…
Amy Lum is selected to present at the Laidlaw Conference at Columbia University
June 11th, 2025
Amy Lum, Julius Benicky, Radoslav Goldman. Generation of Galectin-3-Binding Protein (LG3BP) F357W Mutant as a Crucial Resource for Characterization of Sulf-2/LG3BP…
SULF1+ CAF support progression of colorectal cancer
June 11th, 2025
Cancer-Associated Fibroblasts Expressing Sulfatase 1 Facilitate VEGFA-Dependent Microenvironmental Remodeling to Support Colorectal Cancer. Wang H, Chen J, Chen X, Liu Y, Wang J, Meng Q, Wang H, He…
SULFs regulate bone marrow hematopoiesis
June 11th, 2025
Myeloid deficiency of heparan sulfate 6-O-endosulfatases impairs bone marrow hematopoiesis. Whitehead AK, Wang Z, Boustany RJ, Vivès RR, Lazartigues E, Liu J, Siggins RW, Yue X. Matrix Biol. 2024…
A nice paper showing how PDX engraftment predict survival of OSCC patients
June 11th, 2025
Molecular correlates for HPV-negative head and neck cancer engraftment prognosticate patient outcomes. Waas M, Karamboulas C, Wu BZ, Khan S, Poon S, Meens J, Govindarajan M, Khoo A, Mejia-Guerrero…
Our collaboration with Glycan Therapeutics on MS quantification of SULF activity is selected a Glycobiology Editor’s Choice publication
June 11th, 2025
Development of a method to measure the activity of heparan sulfate 6-endosulfatase for biological research. Wang Z, Benicky J, Mukherjee P, Laing J, Xu Y, Pagadala V, Wu S, Hippensteel JA, Goldman…
Dr. Panigrahi et al. describe an LCM-LC-MS/MS method for spatially resolved proteomics of archived FFPE tissues of OSCC patients
June 11th, 2025
dia-PASEF Proteomics of Tumor and Stroma LMD Enriched from Archived HNSCC Samples. Panigrahi A, Hunt AL, Assis D, Willetts M, Kallakury BV, Davidson B, Ahn J, Conrads TP, Goldman R. ACS Omega. 2025…
Our collaboration with Dr. Pomin et al. shows how glycan polymers inhibit Sulf-2 activity
June 11th, 2025
Heparan-6-O-endosulfatase 2, a cancer-related proteoglycan enzyme, is effectively inhibited by a specific sea cucumber fucosylated glycosaminoglycan. Farrag M, Aljuhani R, Benicky J, Al Ahmed H,…
Congrats to Dr. Mukherjee on a study showing SULF2-mediated invasion of HNSCC cells co-cultured with primary CAF
June 11th, 2025
Heparan-6-O-Endosulfatase 2 Promotes Invasiveness of Head and Neck Squamous Carcinoma Cell Lines in Co-Cultures with Cancer-Associated Fibroblasts. Mukherjee P, Zhou X, Benicky J, Panigrahi A,…
Our collaborative paper with Glycan Therapeutics on MS analysis of SULF activity is a Glycobiology Editor’s Choice
June 11th, 2025
Development of a method to measure the activity of heparan sulfate 6-endosulfatase for biological research. Wang Z, Benicky J, Mukherjee P, Laing J, Xu Y, Pagadala V, Wu S, Hippensteel JA, Goldman…
Our collaboration with colleagues at the University of Mississippi reveals how glycan polymers inhibit Sulf-2 activity
June 11th, 2025
Galectin-3-binding protein inhibits extracellular heparan 6-O-endosulfatse Sulf-2. Panigrahi A, Benicky J, Aljuhani R, Mukherjee P, Nováková Z, Bařinka C, Goldman R. bioRxiv. 2023 Dec…
Congrats to Dr. Panigrahi & colleagues on a spatially resolved proteomics study of FFPE tissues of HNSCC patients
June 11th, 2025
dia-PASEF Proteomics of Tumor and Stroma LMD Enriched from Archived HNSCC Samples. Panigrahi A, Hunt AL, Assis D, Willetts M, Kallakury BV, Davidson B, Ahn J, Conrads TP, Goldman R. ACS Omega. 2025…
SULFs in cancer metastasis
June 25th, 2024
6-O-endosulfatases in tumor metastasis: heparan sulfate proteoglycans modification and potential therapeutic targets. Han M, Zhu H, Chen X, Luo X. Am J Cancer Res. 2024 Feb 25;14(2):897-916. doi:…
Glycoproteins and cancer-stromal cell interactions mediate resistance to HER2-targeting therapies
June 25th, 2024
Cancer-stromal cell interactions in breast cancer brain metastases induce glycocalyx-mediated resistance to HER2-targeting therapies. Goyette MA, Stevens LE, DePinho CR, Seehawer M, Nishida J, Li Z,…
Mucin-selective proteolytic degradation
June 25th, 2024
Design of a mucin-selective protease for targeted degradation of cancer-associated mucins. Pedram K, Shon DJ, Tender GS, Mantuano NR, Northey JJ, Metcalf KJ, Wisnovsky SP, Riley NM, Forcina GC,…
A review of glycosaminoglycan biology
June 25th, 2024
A biological guide to glycosaminoglycans: current perspectives and pending questions. Ricard-Blum S, Vivès RR, Schaefer L, Götte M, Merline R, Passi A, Heldin P, Magalhães A, Reis CA, Skandalis…
Congrats to Dr. Pritha Mukherjee on a paper on inhibitors of HNSCC cell invasion
June 25th, 2024
Aspartate β-Hydroxylase Is Upregulated in Head and Neck Squamous Cell Carcinoma and Regulates Invasiveness in Cancer Cell Models. Mukherjee P, Zhou X, Galli S, Davidson B, Zhang L, Ahn J, Aljuhani…
Congrats to Amy Lum
June 25th, 2024
Congrats to Amy Lum, undergraduate student researcher, on receipt of a Laidlaw scholarship supporting her research at the…
A novel way to model protein-carbohydrate interactions
June 25th, 2024
Highly accurate carbohydrate-binding site prediction with DeepGlycanSite. Xinheng He, Lifen Zhao, Yinping Tian, Rui Li, Qinyu Chu, Zhiyong Gu, Mingyue Zheng, Yusong Wang, Shaoning Li, Hualiang…
Congrats to Dr. Aswini Panigrahi on a paper showing SULF2 inhibition by galectin-3-binding protein
June 25th, 2024
Galectin-3-binding protein inhibits extracellular heparan 6-O-endosulfatase Sulf-2. Panigrahi A, Benicky J, Aljuhani R, Mukherjee P, Nováková Z, Bařinka C, Goldman R. Mol Cell Proteomics. 2024…
Our proteomics study of OSCC survival will be presented at the 2024 ASMS meeting in Anaheim
February 18th, 2024
A study of LCM microdissected tumors analyzing proteins in tumor epithelial and stroma fractions associated with node positivity and poor survival of HNSCC patients. DIA-PASEF proteomic analysis of…
Our paper on the regulation of SULF2 activity by galectin3 binding protein is now under review
February 12th, 2024
Galectin-3-binding protein inhibits extracellular heparan 6-O-endosulfatse Sulf-2. Panigrahi A, Benicky J, Aljuhani R, Mukherjee P, Nováková Z, Bařinka C, Goldman R. bioRxiv. 2023 Dec…
Congrats to Reem Aljuhani on a travel award to present a poster at the ASBMB meeting in San Antonio
February 12th, 2024
Purification and in vitro Characterization of Human Extracellular Heparan 6-O-Endo-Sulfatases Sulf1 and Sulf2. Aljuhani R, Benicky J, Zhang L, Liu J, Pomin VH, Goldman R. ASBMB San Antonio, March…
Drs. Benicky and Panigrahi present at the 2023 Annual SfG meeting in Hawaii
February 12th, 2024
A 6-O-endosulfatase Activity Assay Based on Synthetic Heparan Sulfate Oligomers. Benicky J, Liu J, Wang Z, Pagadala V, Panigrahi A, Sanda M, Su G, and Goldman R. Society for Glycobiology Annual…
Congrats to Dr. Mukherjee on a study showing SULF2-mediated invasion of HNSCC cells co-cultured with primary CAF
February 12th, 2024
Heparan-6-O-Endosulfatase 2 Promotes Invasiveness of Head and Neck Squamous Carcinoma Cell Lines in Co-Cultures with Cancer-Associated Fibroblasts. Mukherjee P, Zhou X, Benicky J, Panigrahi A,…
Dr. Panigrahi et al. share a preprint on the regulation of SULF2 activity by Galectin3 binding protein in BioRxiv
February 12th, 2024
Galectin-3-binding protein inhibits extracellular heparan 6-O-endosulfatse Sulf-2. Panigrahi A, Benicky J, Aljuhani R, Mukherjee P, Nováková Z, Bařinka C, Goldman R. bioRxiv. 2023 Dec…
SULF2 is a target gene regulated by TGFb via LINC00313 in cholangiocarcinoma
February 12th, 2024
TGFβ-induced long non-coding RNA LINC00313 activates Wnt signaling and promotes cholangiocarcinoma. Papoutsoglou P, Pineau R, Leroux R, Louis C, L’Haridon A, Foretek D, Morillon A, Banales JM,…
SULF1 positive fibroblast regulate lizard tail regeneration induced by hedgehog signaling
February 12th, 2024
Single-cell analysis of lizard blastema fibroblasts reveals phagocyte-dependent activation of Hedgehog-responsive chondrogenesis. Vonk AC, Zhao X, Pan Z, Hudnall ML, Oakes CG, Lopez GA,…
Structure-function of an exostosin heterodimer (EXT1 and EXT2) that extends HSPG polymers
February 12th, 2024
Structural basis for heparan sulfate co-polymerase action by the EXT1-2 complex. Li H, Chapla D, Amos RA, Ramiah A, Moremen KW, Li H. Nat Chem Biol. 2023 May;19(5):565-574. doi:…
EXTL3 selects heparan sulfate synthesis over chondroitin sulfation of glycosaminoglycans
February 12th, 2024
Molecular mechanism of decision-making in glycosaminoglycan biosynthesis. Sammon D, Krueger A, Busse-Wicher M, Morgan RM, Haslam SM, Schumann B, Briggs DC, Hohenester E. Nat Commun. 2023 Oct…
Our paper on SULF2-promoted invasiveness of HNSCC just published
October 26th, 2023
Our paper “Heparan-6-O-endosulfatase 2 promotes invasiveness of head and neck squamous carcinoma cell lines in co-cultures with cancer associated fibroblasts” by Pritha Mukherjee, Xin Zhou,…
Josh Tu joins the CTGRC for his PhD research rotation
October 15th, 2023
Josh is a first year PhD student of Biochemistry and Molecular & Cellular Biology. He studies inhibition of the heparan 6-O-endosulfatase enzymes by marine fucosylated chondroitins as part of…
Reem Aljuhani presents her studies of heparan-6-O-endosulfatatses at a BMCB seminar series
October 15th, 2023
Reem presents her discoveries on the “Expression and characterization of extracellular heparan 6-O-endosulfatases Sulf1 and Sulf2” at the Biochemistry and Molecular & Cellular Biology (BMCB)…
New details on the function of N-glycans in the FcγRIIIa interaction with IgG
October 15th, 2023
A paper by Van Coillie et al. shows that afucosylated N-glycans of IgG have highest affinity for the oligomannose FcγRIIIa, a glycan structure commonly found on Asn162 on FcγRIIIa expressed by NK…
Dr. Panigrahi presents a poster on the detection of liver fibrosis at the 2023 SFG meeting in Hawaii
October 15th, 2023
His poster “Serologic LC-MS/MS-PRM of IgG N-glycoform G0FN and sialylated HPX O-glycoforms effectively identifies progressing liver fibrosis” is co-authored byAswini Panigrahi1,2, Lihua…
Dr. Benicky presents a poster on SULF activity measurements at the 2023 SFG meeting in Hawaii
October 15th, 2023
His poster “A 6-O-endosulfatase Activity Assay Based on Synthetic Heparan Sulfate Oligomers” is co-authored by Julius Benicky1, Jian Liu2, Zhangjie Wang3, Vijayakanth Pagadala3, Aswini…
Discovery of global inhibitors of cellular glycosylation
October 15th, 2023
A paper by Sorensen et al. identifies two compounds that mediate reversible fragmentation of the Golgi system without affecting secretion; this leads to expression of truncated O-glycans or to the…
SULF2 derives mainly from cancer associated fibroblasts in hepatocellular cancers
October 15th, 2023
A paper by Zaki et al. shows that SULF2 is produced in part by cancer cells but that majority of the SULF2 enzyme is supplied by cancer assicated fibroblasts which correlates with poor prognosis.…
Dr. Mukherjee et al. share a preprint on the SULF2-supported invasion of HNSC cells in BioRxiv
October 15th, 2023
The paper “Heparan-6-O-endosulfatase 2 promotes invasiveness of head and neck squamous carcinoma cell lines in co-cultures with cancer associated fibroblasts” by Pritha Mukherjee, Xin Zhou,…
An update on the glycoproteomics of the TIM family of immune checkpoint inhibitors
October 15th, 2023
A paper by Chongsaritsinsuk et al. expose mucin-type O-glycosylation of the T cell immunoglobulin and mucin-domain containing family of proteins (TIMs) and their structure-function models.…
Congrats to Dr. Benicky on a paper on the quantification of SULF activity
July 13th, 2023
A 6-O-endosulfatase activity assay based on synthetic heparan sulfate oligomers. Benicky J, Sanda M, Panigrahi A, Liu J, Wang Z, Pagadala V, Su G, Goldman R. Glycobiology. 2023 33(5):384-395. doi:…
SULF2 is supplied to HCC tissues by cancer associated fibroblasts
July 13th, 2023
Sulfatase-2 from Cancer Associated Fibroblasts: An Environmental Target for Hepatocellular Carcinoma? Zaki MYW, Alhasan SF, Shukla R, McCain M, Laszczewska M, Geh D, Patman GL, Televantou D,…
SULF2, GPC3, and SDC1 in serum predict poor outcomes in HCC
July 13th, 2023
Sulfatase 2 Along with Syndecan 1 and Glypican 3 Serum Levels are Associated with a Prognostic Value in Patients with Alcoholic Cirrhosis-Related Advanced Hepatocellular Carcinoma. Mouhoubi N,…
Regulation of heparan sulfate assembly by TFCP2
July 13th, 2023
TFCP2 is a transcriptional regulator of heparan sulfate assembly and melanoma cell growth. Basu A, Champagne RN, Patel NG, Nicholson ED, Weiss RJ. J Biol Chem. 2023 Jun;299(6):104713. doi:…
An update on fragmentation of glycopeptides from Dr. Sanda’s group
July 13th, 2023
HCC EV ECG score: An extracellular vesicle-based protein assay for detection of early-stage hepatocellular carcinoma. Sun N, Zhang C, Lee YT, Tran BV, Wang J, Kim H, Lee J, Zhang RY, Wang JJ, Hu J,…
Congrats to Drs. Sanda and Panigrahi on significantly improved LC-MS/MS quantification of glycopeptides
February 24th, 2023
LC-MS/MS-PRM Quantification of IgG Glycoforms Using Stable Isotope Labeled IgG1 Fc Glycopeptide Standard. Sanda M, Yang Q, Zong G, Chen H, Zheng Z, Dhani H, Khan K, Kroemer A, Wang LX, Goldman R. J…
Dr. Goldman presents on Heparan 6-O-endosulfatases in cancer diseases
February 23rd, 2023
Dr. Goldman presents on the Heparan 6-O-endosulfatases in cancer diseases at the Czech Annual Cancer Research Meeting in Olomouc, December 1 2022:…
SULF1 is associated with Gleason Score and predicts prostate cancer outcomes
February 23rd, 2023
The Development of a Gleason Score-Related Gene Signature for Predicting the Prognosis of Prostate Cancer. Yimamu Y, Yang X, Chen J, Luo C, Xiao W, Guan H, Wang D. J Clin Med. 2022 Dec…
Congrats to Dr Yang and co-authors on paper summarizing the impact of the heparan 6-O-endosulfatases in HNSC and other cancers
February 23rd, 2023
Extracellular Heparan 6-O-Endosulfatases SULF1 and SULF2 in Head and Neck Squamous Cell Carcinoma and Other Malignancies. Yang Y, Ahn J, Edwards NJ, Benicky J, Rozeboom AM, Davidson B, Karamboulas…
