SULF2 mediates activation of synovial fibroblasts in rheumatoid arthritis
October 19th, 2022
Extracellular sulfatase-2 is overexpressed in rheumatoid arthritis and mediates the TNF-α-induced inflammatory activation of synovial fibroblasts. Siegel RJ, Singh AK, Panipinto PM, Shaikh FS, Vinh…
Continued search for effective inhibitors of the SULFs
October 19th, 2022
Design and Synthesis of 1-O- and 6′-C-Modified Heparan Sulfate Trisaccharides as Human Endo-6-O-Sulfatase 1 Inhibitors. Tseng KY, Tzeng ZH, Cheng TR, Liang PH, Hung SC. Front Chem. 2022 Jul…
Our updated results on the SULFs
October 19th, 2022
An update on the function of the heparan 6-O-endosulfatases (SULF1 and SULF2) in cancer disease is now under review and deposited on…
Reem Aljuhani successfully defends her Phase 2 PhD exam
October 19th, 2022
Congrats to Reem Aljuhani on a successful Phase 2 exam in her PhD program!! She presented a paper on “Heparan sulfate binding proteins dependent on 6-O-sulfation in Head and neck squamous cells…
Dr. Goldman expands collaborations with scientist at the Czech National Academy of Sciences during his sabbatical
October 19th, 2022
The studies target analysis of the structure and function of the heparan 6-O-endosulfatases and are designed to generate inhibitors and antibodies specific to these important…
A collaboration with Glycan Therapeutics
October 19th, 2022
We will be starting soon a collaborative SBIR research together with Prof. Jian Liu and his colleagues at Glycan Therapeutics. The award examines synthetic probes for the activity of the…
Chemistry Nobel Prize 2022
October 19th, 2022
Sincere congrats to Carolyn R. Bertozzi on her Nobel Prize shared with Morten P. Medal and K. Barry Sharpless!! Carolyn walks with grace the boundary of chemistry and biology and she continues to…
Dr. Edwards presents at the Common Fund Glycoscience Investigator Meeting
July 5th, 2022
Dr. Edwards presents “LC-MS Analysis of Site-specific Protein Glycoforms” and “Bridging the Gap: Connecting Glycobiology and Glycoinformatics” at the Common Fund Glycoscience Investigator…
Dr. Panigrahi presents at 2022 NIH & FDA Glycoscience Research Day
July 5th, 2022
Dr. Panigrahi presented a poster “Microflow LC-MS/MS-PRM Quantification of Protein Glycoforms: Serologic Assays of Liver Fibrosis” at the 2022 NIH & FDA Glycoscience Research Day June 13th…
Dr. Benicky receives award at 2022 NIH & FDA Glycoscience Research Day
July 5th, 2022
Dr. Benicky received an award for his poster “Purification and characterization of human recombinant extracellular 6-O-endosulfatases” at the 2022 NIH & FDA Glycoscience Research Day June…
Dr. Yang Yang graduates and joins Novartis
April 8th, 2022
Congrats to Dr. Yang Yang for her postdoctoral fellowship with Novartis in San Diego.…
Dr. Edwards presents on GNOme at the International Conference on Biomedical Ontology 2021
April 8th, 2022
“GNOme – Glycan Naming and Subsumption Ontology”, with Wenjin Zhang. In Proceedings of the International Conference on Biomedical Ontologies 2021,…
Dr. Goldman presents at the “Glycoinformatics at the Interface of Disease and Data” virtual conference organized by the NIH Common Fund’s Tools for Glycoscience Program
April 8th, 2022
“Heparan 6-O-endosulfatases in HNSCC and Other Cancers” lecture will be presented by Rado Goldman on April 27 in the Cancer Glycobiology section…
Researchers confirm that human SULF2 is a cancer-promoting chondroitin sulfate proteoglycan
April 8th, 2022
Extracellular endosulfatase Sulf-2 harbors a chondroitin/dermatan sulfate chain that modulates its enzyme activity. El Masri R, Seffouh A, Roelants C, Seffouh I, Gout E, Pérard J, Dalonneau F,…
Synthetic isotopically labeled standards improve quantification of heparan sulfation
April 8th, 2022
Analysis of 3-O-Sulfated Heparan Sulfate Using Isotopically Labeled Oligosaccharide Calibrants. Wang Z, Arnold K, Dhurandahare VM, Xu Y, Pagadala V, Labra E, Jeske W, Fareed J, Gearing M, Liu J.…
Glycan-dependent interactions of SARS CoV2
April 8th, 2022
Host Cell Glycocalyx Remodeling Reveals SARS-CoV-2 Spike Protein Glycomic Binding Sites. Ying Sheng, Anita Vinjamuri, Michael Russelle S. Alvarez, Yixuan Xie, Marisa McGrath, Siyu Chen, Mariana…
Dr. Goldman presents at US HUPO 2022
April 8th, 2022
“Heparan Sulfate 6-O-endosulfatases in HNSCC and Other Malignancies” talk by Rado Goldman at the US HUPO 2022 18th Annual Conference in Charleston, SC February 28 in Parallel Session 4:…
Collagen organization regulates tumor progression
April 8th, 2022
A tumor-derived type III collagen-rich ECM niche regulates tumor cell dormancy. Di Martino JS, Nobre AR, Mondal C, Taha I, Farias EF, Fertig EJ, Naba A, Aguirre-Ghiso JA, Bravo-Cordero JJ. Nat…
Collagen regulates tumors by immunosuppression
April 8th, 2022
Tumour DDR1 promotes collagen fibre alignment to instigate immune exclusion. Sun X, Wu B, Chiang HC, Deng H, Zhang X, Xiong W, Liu J, Rozeboom AM, Harris BT, Blommaert E, Gomez A, Garcia RE, Zhou Y,…
Dr. Panigrahi co-authors a Nature Communications proteogenomic study of neoantigens
January 3rd, 2022
Proteogenomic discovery of neoantigens facilitates personalized multi-antigen targeted T cell immunotherapy for brain tumors. Rivero-Hinojosa S, Grant M, Panigrahi A, Zhang H, Caisova V, Bollard CM,…
More evidence that glycosylkation affects SARS-CoV-2 infectivity
January 3rd, 2022
SARS-Cov-2 Spike binding to ACE2 is stronger and longer ranged due to glycan interaction. Huang Y, Harris BS, Minami SA, Jung S, Shah PS, Nandi S, McDonald KA, Faller R Biophysical Journal (2022)…
Non-redundant function of 6-O-endosulfatases in the developing spinal cord
December 9th, 2021
Sulf2a controls Shh-dependent neural fate specification in the developing spinal cord. Danesin C, Darche-Gabinaud R, Escalas N, Bouguetoch V, Cochard P, Al Oustah A, Ohayon D, Glise B, Soula C Sci…
Inhibition of 6-O-endosulfatases accelerates oligodendrocyte recruitment and remyelination
December 9th, 2021
Overcoming the inhibitory microenvironment surrounding oligodendrocyte progenitor cells following experimental demyelination. Saraswat D, Shayya HJ, Polanco JJ, Tripathi A, Welliver RR, Pol SU,…
O-glycosylation regulates furin cleavage of the SARS-CoV-2 spike
December 9th, 2021
Furin cleavage of the SARS-CoV-2 spike is modulated by O-glycosylation. Zhang L, Mann M, Syed ZA, Reynolds HM, Tian E, Samara NL, Zeldin DC, Tabak LA, Ten Hagen KG. Proc Natl Acad Sci U S A. 2021…
Targeting evolutionarily-conserved N-glycosylation affects SARS-CoV-2 infectivity
December 9th, 2021
Targeting conserved N-glycosylation blocks SARS-CoV-2 variant infection in vitro. Hsiang-Chi Huang, Yun-Ju Lai, Chun-Che Liao, Wang-Feng Yang, Ke-Bin Huang, I-Jung Lee, Wen-Cheng Chou, Shih-Han…
Dr Edwards presents on GNOme ontology at ICBO 2021
October 5th, 2021
“GNOme – Glycan Naming and Subsumption Ontology” Wenjin Zhang and Nathan Edwards at the 12th International Conference on Biomedical Ontologies (2021).…
Dr Sanda presents November 2nd on the quantification of IgG glycoforms at the 69th ASMS Conference
October 5th, 2021
“Inter- and intra-scan normalization for optimized LC-MS-PRM workflow of IgG glycosylation measurement.” Miloslav Sanda, Qiang Yang, Lai-xi Wang, Radoslav Goldman This collaborative study…
CTGRC researchers publish a collaborative study of PSMA glycoforms
October 5th, 2021
“Glycoforms of human Prostate-specific membrane antigen (PSMA) in human cells and prostate tissue” Wei Yuan, Baoqin Liu, Miloslav Sanda, Renhuizi Wei, Julius Benicky, Zora Novakova, Cyril Barinka,…
A study of glycoproteomics tools is accepted in Nature Methods
October 5th, 2021
“Community Evaluation of Glycoproteomics Informatics Solutions Reveals High-Performance Search Strategies of Serum N- and O-Glycopeptide Data”, Rebeca Kawahara, Anastasia Chernykh, Kathirvel…
Dr. Aswini Panigrahi joins CTGRC
October 5th, 2021
Dr. Panigrahi joins the analytical group to conduct mass spectrometric studies of glycoconjugates.…
CTGRC receives funds to install timsToF Pro mass spectrometer
October 5th, 2021
NIH awards S10OD028623 to CTGRC researchers to study ion-mobility assisted LC-MS/MS workflows for the quantification of glycopeptides, as well as structural resolution of glycoforms, using the…
CTGRC Announces Glycopeptide DIA Interlaboratory Study
July 7th, 2021
Dr. Miloslav Sanda, Director of Analytical Chemistry at the CTGRC, announced the Glycopeptide DIA Interlaboratory Study today. Please contact Mila (ms2465@goergetown.edu) if you are interested in…
Sialic acid, in addition to heparan sulfate, mediates viral entry of SARS-CoV-2
June 30th, 2021
Sialic Acid-Dependent Binding and Viral Entry of SARS-CoV-2. Linh Nguyen, Kelli A. McCord, Duong T. Bui, Kim M. Bouwman, Elena N. Kitova, Dhanraj Kumawat, Gour C. Daskhan, Ilhan Tomris, Ling Han,…
Glycans in SARS-CoV-2 antibody production
May 7th, 2021
Glycans of SARS-CoV-2 Spike Protein in Virus Infection and Antibody Production. Xiaohui Zhao, Huan Chen, Hongliang Wang. Front Mol Biosci 2021 Apr 13;8:629873. eCollection 2021. doi:…
CTGRC Virtual Posters at the 2021 NIH & FDA Glycoscience Research Day on June 1st
May 7th, 2021
Conference website and registration. “Disialo T antigen in the progression of liver disease”, Miloslav Sanda, Julius Benicky, and Radoslav Goldman. “Purification and characterization of…
Therapeutic implications of the inhibition of SULF2
May 7th, 2021
SULF2 monoclonal antibody 5D5 suppresses human cholangiocarcinoma xenograft growth via regulation of a SULF2-PDGFRβ-YAP signaling axis. Luo X, Campbell NA, He L, O’Brien DR, Singer MS,…
Mucin domain-targeting proteases open new avenues in mucin research
May 7th, 2021
Classification, structural biology, and applications of mucin domain-targeting proteases. Shon DJ, Kuo A, Ferracane MJ, Malaker SA. Biochem J. 2021 Apr 30;478(8):1585-1603. doi:…
A nice study of SARS-CoV-2 binding to heparan sulfate
May 7th, 2021
Preferential recognition and antagonism of SARS-CoV-2 spike glycoprotein binding to 3-O-sulfated heparan sulfate. Vaibhav Tiwari, Ritesh Tandon, Nehru Viji Sankaranarayanan, Jacob C. Beer, Ellen K.…
Advances in the studies of SARS-CoV-2 O-glycosylation
May 7th, 2021
Altered O-glycosylation Level of SARS-CoV-2 Spike Protein by Host O-glycosyltransferase Strengthens Its Trimeric Structure. Zhijue Xu, Xin Ku, Jiaqi Tian, Han Zhang, Jingli Hou, Can Zhang, Jingjing…
A nice summary of Glycosaminoglycan–Protein Interactions
May 7th, 2021
Glycosaminoglycan-Protein Interactions: The First Draft of the Glycosaminoglycan Interactome. Vallet SD, Clerc O, Ricard-Blum S. J Histochem Cytochem. 2021 Feb;69(2):93-104. doi:…
A nice development in SULF-2 research: it is a proteoglycan
April 12th, 2021
“Extracellular endosulfatase Sulf-2 harbours a chondroitin/dermatan sulfate chain that modulates its enzyme activity” Rana El Masri, Amal Seffouh, Caroline Roelants, Ilham Seffouh, Evelyne Gout,…
A paper on the glycopeptides of SARS CoV-2 displayed by MHCII to be published soon in Cell Reports
April 12th, 2021
Mapping the SARS-CoV-2 spike glycoprotein-derived peptidome presented by HLA class II on dendritic cells. Parker R, Partridge T, Wormald C, Kawahara R, Stalls V, Aggelakopoulou M, Parker J, Doherty…
Sialic acid on ACE2 and heparan sulfation modify SARS-CoV-2 infectivity
April 12th, 2021
Host and viral determinants for efficient SARS-CoV-2 infection of the human lung. Chu H, Hu B, Huang X, Chai Y, Zhou D, Wang Y, Shuai H, Yang D, Hou Y, Zhang X, Yuen TT, Cai JP, Zhang AJ, Zhou J,…
Dr. Sanda collaborates with SCIEX researchers on CESI TripleTOF LC-MS/MS analysis of Adalimumab glycoforms
April 12th, 2021
The results were published in a Sciex release (https://sciex.com/content/dam/SCIEX/pdf/tech-notes/all/CESI_mAb.pdf) and presented by Mila at the US HUPO 2021 conference, virtual, 03/08/2021:…
An elegant method for selective de-glycosylation of O-GlcNAcylated proteins in cells with application to transcriptional regulation of c-Jun and c-Fos
April 12th, 2021
Target protein deglycosylation in living cells by a nanobody-fused split O-GlcNAcase. Ge Y, Ramirez DH, Yang B, D’Souza AK, Aonbangkhen C, Wong S, Woo CM. Nat Chem Biol. 2021 Mar 8. doi:…
CTGRC researchers participate in an inter-laboratory glycoproteomics study in review at Nature Methods
April 12th, 2021
“Community Evaluation of Glycoproteomics Informatics Solutions Reveals High-Performance Search Strategies of Glycopeptide Data.” Rebeca Kawahara, Kathirvel Alagesan, Marshall Bern, Weiqian Cao,…
A new report on lysosomal exit of SARS CoV-2 from infected cells
December 10th, 2020
“β-Coronaviruses Use Lysosomes for Egress Instead of the Biosynthetic Secretory Pathway” Sourish Ghosh, Teegan A Dellibovi-Ragheb, Adeline Kerviel, Eowyn Pak, Qi Qiu, Matthew Fisher, Peter M…
An update on the S1 glycoforms of the SARS-CoV-2 released by CALU-3 cells
December 10th, 2020
“Analysis of SARS-CoV-2 spike glycosylation reveals shedding of a vaccine candidate.” Juliane Brun, Snežana Vasiljevic, Bevin Gangadharan, Mario Hensen, Anu V. Chandran, Michelle L. Hill,…
Yang Yang has a new publication on heparan sulfation in Frontiers in Oncology
December 10th, 2020
Yang’s paper “Expression of the Extracellular Sulfatase SULF2 Affects Survival of Head and neck Squamous Cell Carcinoma Patients” was just accepted…
Dr. Miloslav Sanda (CTGRC Director of Analytical Chemistry) will present at Stanford
October 23rd, 2020
Mila presents on “Structure and Site-specific N- and O- Glycosylation of the SARS-CoV-2 Spike Glycoprotein using Low Collision Energy Fragmentation and Cyclic Ion Mobility” at the upcoming…
