Our paper on SULF2-promoted invasiveness of HNSCC just published
October 26th, 2023
Our paper “Heparan-6-O-endosulfatase 2 promotes invasiveness of head and neck squamous carcinoma cell lines in co-cultures with cancer associated fibroblasts” by Pritha Mukherjee, Xin Zhou,…
Josh Tu joins the CTGRC for his PhD research rotation
October 15th, 2023
Josh is a first year PhD student of Biochemistry and Molecular & Cellular Biology. He studies inhibition of the heparan 6-O-endosulfatase enzymes by marine fucosylated chondroitins as part of…
Reem Aljuhani presents her studies of heparan-6-O-endosulfatatses at a BMCB seminar series
October 15th, 2023
Reem presents her discoveries on the “Expression and characterization of extracellular heparan 6-O-endosulfatases Sulf1 and Sulf2” at the Biochemistry and Molecular & Cellular Biology (BMCB)…
New details on the function of N-glycans in the FcγRIIIa interaction with IgG
October 15th, 2023
A paper by Van Coillie et al. shows that afucosylated N-glycans of IgG have highest affinity for the oligomannose FcγRIIIa, a glycan structure commonly found on Asn162 on FcγRIIIa expressed by NK…
Dr. Panigrahi presents a poster on the detection of liver fibrosis at the 2023 SFG meeting in Hawaii
October 15th, 2023
His poster “Serologic LC-MS/MS-PRM of IgG N-glycoform G0FN and sialylated HPX O-glycoforms effectively identifies progressing liver fibrosis” is co-authored byAswini Panigrahi1,2, Lihua…
Dr. Benicky presents a poster on SULF activity measurements at the 2023 SFG meeting in Hawaii
October 15th, 2023
His poster “A 6-O-endosulfatase Activity Assay Based on Synthetic Heparan Sulfate Oligomers” is co-authored by Julius Benicky1, Jian Liu2, Zhangjie Wang3, Vijayakanth Pagadala3, Aswini…
Discovery of global inhibitors of cellular glycosylation
October 15th, 2023
A paper by Sorensen et al. identifies two compounds that mediate reversible fragmentation of the Golgi system without affecting secretion; this leads to expression of truncated O-glycans or to the…
SULF2 derives mainly from cancer associated fibroblasts in hepatocellular cancers
October 15th, 2023
A paper by Zaki et al. shows that SULF2 is produced in part by cancer cells but that majority of the SULF2 enzyme is supplied by cancer assicated fibroblasts which correlates with poor prognosis.…
Dr. Mukherjee et al. share a preprint on the SULF2-supported invasion of HNSC cells in BioRxiv
October 15th, 2023
The paper “Heparan-6-O-endosulfatase 2 promotes invasiveness of head and neck squamous carcinoma cell lines in co-cultures with cancer associated fibroblasts” by Pritha Mukherjee, Xin Zhou,…
An update on the glycoproteomics of the TIM family of immune checkpoint inhibitors
October 15th, 2023
A paper by Chongsaritsinsuk et al. expose mucin-type O-glycosylation of the T cell immunoglobulin and mucin-domain containing family of proteins (TIMs) and their structure-function models.…
Congrats to Dr. Benicky on a paper on the quantification of SULF activity
July 13th, 2023
A 6-O-endosulfatase activity assay based on synthetic heparan sulfate oligomers. Benicky J, Sanda M, Panigrahi A, Liu J, Wang Z, Pagadala V, Su G, Goldman R. Glycobiology. 2023 33(5):384-395. doi:…
SULF2 is supplied to HCC tissues by cancer associated fibroblasts
July 13th, 2023
Sulfatase-2 from Cancer Associated Fibroblasts: An Environmental Target for Hepatocellular Carcinoma? Zaki MYW, Alhasan SF, Shukla R, McCain M, Laszczewska M, Geh D, Patman GL, Televantou D,…
SULF2, GPC3, and SDC1 in serum predict poor outcomes in HCC
July 13th, 2023
Sulfatase 2 Along with Syndecan 1 and Glypican 3 Serum Levels are Associated with a Prognostic Value in Patients with Alcoholic Cirrhosis-Related Advanced Hepatocellular Carcinoma. Mouhoubi N,…
Regulation of heparan sulfate assembly by TFCP2
July 13th, 2023
TFCP2 is a transcriptional regulator of heparan sulfate assembly and melanoma cell growth. Basu A, Champagne RN, Patel NG, Nicholson ED, Weiss RJ. J Biol Chem. 2023 Jun;299(6):104713. doi:…
An update on fragmentation of glycopeptides from Dr. Sanda’s group
July 13th, 2023
HCC EV ECG score: An extracellular vesicle-based protein assay for detection of early-stage hepatocellular carcinoma. Sun N, Zhang C, Lee YT, Tran BV, Wang J, Kim H, Lee J, Zhang RY, Wang JJ, Hu J,…
Congrats to Drs. Sanda and Panigrahi on significantly improved LC-MS/MS quantification of glycopeptides
February 24th, 2023
LC-MS/MS-PRM Quantification of IgG Glycoforms Using Stable Isotope Labeled IgG1 Fc Glycopeptide Standard. Sanda M, Yang Q, Zong G, Chen H, Zheng Z, Dhani H, Khan K, Kroemer A, Wang LX, Goldman R. J…
Dr. Goldman presents on Heparan 6-O-endosulfatases in cancer diseases
February 23rd, 2023
Dr. Goldman presents on the Heparan 6-O-endosulfatases in cancer diseases at the Czech Annual Cancer Research Meeting in Olomouc, December 1 2022:…
SULF1 is associated with Gleason Score and predicts prostate cancer outcomes
February 23rd, 2023
The Development of a Gleason Score-Related Gene Signature for Predicting the Prognosis of Prostate Cancer. Yimamu Y, Yang X, Chen J, Luo C, Xiao W, Guan H, Wang D. J Clin Med. 2022 Dec…
Congrats to Dr Yang and co-authors on paper summarizing the impact of the heparan 6-O-endosulfatases in HNSC and other cancers
February 23rd, 2023
Extracellular Heparan 6-O-Endosulfatases SULF1 and SULF2 in Head and Neck Squamous Cell Carcinoma and Other Malignancies. Yang Y, Ahn J, Edwards NJ, Benicky J, Rozeboom AM, Davidson B, Karamboulas…
An interesting paper on urinary and plasma GAGs in cancer detection
February 23rd, 2023
Noninvasive detection of any-stage cancer using free glycosaminoglycans. Bratulic S, Limeta A, Dabestani S, Birgisson H, Enblad G, Stålberg K, Hesselager G, Häggman M, Höglund M, Simonson OE,…
Congrats to Panigrahi on two papers advancing microflow LC-MS/MS-PRM and the detection of liver fibrosis
February 23rd, 2023
A multiplexed microflow LC-MS/MS-PRM assay for serologic quantification of IgG N- and HPX O- glycoforms in liver fibrosis. Panigrahi A, Zhang L, Benicky J, Sanda M, Ahn J, Goldman R. Sci Rep. 2023…
SULF1 downregulation by EZH2 in chondrosarcoma leads to poor outcomes
February 23rd, 2023
EZH2/hSULF1 axis mediates receptor tyrosine kinase signaling to shape cartilage tumor progression. Lin ZS, Chung CC, Liu YC, Chang CH, Liu HC, Liang YY, Huang TL, Chen TM, Lee CH, Tang CH, Hung MC,…
Dr. Edwards publishes on glycan vocabulary standardization
February 23rd, 2023
The glycan structure dictionary-a dictionary describing commonly used glycan structure terms. Vora J, Navelkar R, Vijay-Shanker K, Edwards N, Martinez K, Ding X, Wang T, Su P, Ross K, Lisacek F,…
A nice paper on an exosome-based detection of HCC
February 23rd, 2023
HCC EV ECG score: An extracellular vesicle-based protein assay for detection of early-stage hepatocellular carcinoma. Sun N, Zhang C, Lee YT, Tran BV, Wang J, Kim H, Lee J, Zhang RY, Wang JJ, Hu J,…
Congrats to Dr. Sanda on the first use of a SIS glycopeptide MS assay for the quantification of IgG glycoforms
February 23rd, 2023
LC-MS/MS-PRM Quantification of IgG Glycoforms Using Stable Isotope Labeled IgG1 Fc Glycopeptide Standard. Sanda M, Yang Q, Zong G, Chen H, Zheng Z, Dhani H, Khan K, Kroemer A, Wang LX, Goldman R. J…
SULF2 mediates activation of synovial fibroblasts in rheumatoid arthritis
October 19th, 2022
Extracellular sulfatase-2 is overexpressed in rheumatoid arthritis and mediates the TNF-α-induced inflammatory activation of synovial fibroblasts. Siegel RJ, Singh AK, Panipinto PM, Shaikh FS, Vinh…
Continued search for effective inhibitors of the SULFs
October 19th, 2022
Design and Synthesis of 1-O- and 6′-C-Modified Heparan Sulfate Trisaccharides as Human Endo-6-O-Sulfatase 1 Inhibitors. Tseng KY, Tzeng ZH, Cheng TR, Liang PH, Hung SC. Front Chem. 2022 Jul…
Our updated results on the SULFs
October 19th, 2022
An update on the function of the heparan 6-O-endosulfatases (SULF1 and SULF2) in cancer disease is now under review and deposited on…
Reem Aljuhani successfully defends her Phase 2 PhD exam
October 19th, 2022
Congrats to Reem Aljuhani on a successful Phase 2 exam in her PhD program!! She presented a paper on “Heparan sulfate binding proteins dependent on 6-O-sulfation in Head and neck squamous cells…
Dr. Goldman expands collaborations with scientist at the Czech National Academy of Sciences during his sabbatical
October 19th, 2022
The studies target analysis of the structure and function of the heparan 6-O-endosulfatases and are designed to generate inhibitors and antibodies specific to these important…
A collaboration with Glycan Therapeutics
October 19th, 2022
We will be starting soon a collaborative SBIR research together with Prof. Jian Liu and his colleagues at Glycan Therapeutics. The award examines synthetic probes for the activity of the…
Chemistry Nobel Prize 2022
October 19th, 2022
Sincere congrats to Carolyn R. Bertozzi on her Nobel Prize shared with Morten P. Medal and K. Barry Sharpless!! Carolyn walks with grace the boundary of chemistry and biology and she continues to…
Dr. Edwards presents at the Common Fund Glycoscience Investigator Meeting
July 5th, 2022
Dr. Edwards presents “LC-MS Analysis of Site-specific Protein Glycoforms” and “Bridging the Gap: Connecting Glycobiology and Glycoinformatics” at the Common Fund Glycoscience Investigator…
Dr. Panigrahi presents at 2022 NIH & FDA Glycoscience Research Day
July 5th, 2022
Dr. Panigrahi presented a poster “Microflow LC-MS/MS-PRM Quantification of Protein Glycoforms: Serologic Assays of Liver Fibrosis” at the 2022 NIH & FDA Glycoscience Research Day June 13th…
Dr. Benicky receives award at 2022 NIH & FDA Glycoscience Research Day
July 5th, 2022
Dr. Benicky received an award for his poster “Purification and characterization of human recombinant extracellular 6-O-endosulfatases” at the 2022 NIH & FDA Glycoscience Research Day June…
Dr. Yang Yang graduates and joins Novartis
April 8th, 2022
Congrats to Dr. Yang Yang for her postdoctoral fellowship with Novartis in San Diego.…
Dr. Edwards presents on GNOme at the International Conference on Biomedical Ontology 2021
April 8th, 2022
“GNOme – Glycan Naming and Subsumption Ontology”, with Wenjin Zhang. In Proceedings of the International Conference on Biomedical Ontologies 2021,…
Dr. Goldman presents at the “Glycoinformatics at the Interface of Disease and Data” virtual conference organized by the NIH Common Fund’s Tools for Glycoscience Program
April 8th, 2022
“Heparan 6-O-endosulfatases in HNSCC and Other Cancers” lecture will be presented by Rado Goldman on April 27 in the Cancer Glycobiology section…
Researchers confirm that human SULF2 is a cancer-promoting chondroitin sulfate proteoglycan
April 8th, 2022
Extracellular endosulfatase Sulf-2 harbors a chondroitin/dermatan sulfate chain that modulates its enzyme activity. El Masri R, Seffouh A, Roelants C, Seffouh I, Gout E, Pérard J, Dalonneau F,…
Synthetic isotopically labeled standards improve quantification of heparan sulfation
April 8th, 2022
Analysis of 3-O-Sulfated Heparan Sulfate Using Isotopically Labeled Oligosaccharide Calibrants. Wang Z, Arnold K, Dhurandahare VM, Xu Y, Pagadala V, Labra E, Jeske W, Fareed J, Gearing M, Liu J.…
Glycan-dependent interactions of SARS CoV2
April 8th, 2022
Host Cell Glycocalyx Remodeling Reveals SARS-CoV-2 Spike Protein Glycomic Binding Sites. Ying Sheng, Anita Vinjamuri, Michael Russelle S. Alvarez, Yixuan Xie, Marisa McGrath, Siyu Chen, Mariana…
Dr. Goldman presents at US HUPO 2022
April 8th, 2022
“Heparan Sulfate 6-O-endosulfatases in HNSCC and Other Malignancies” talk by Rado Goldman at the US HUPO 2022 18th Annual Conference in Charleston, SC February 28 in Parallel Session 4:…
Collagen organization regulates tumor progression
April 8th, 2022
A tumor-derived type III collagen-rich ECM niche regulates tumor cell dormancy. Di Martino JS, Nobre AR, Mondal C, Taha I, Farias EF, Fertig EJ, Naba A, Aguirre-Ghiso JA, Bravo-Cordero JJ. Nat…
Collagen regulates tumors by immunosuppression
April 8th, 2022
Tumour DDR1 promotes collagen fibre alignment to instigate immune exclusion. Sun X, Wu B, Chiang HC, Deng H, Zhang X, Xiong W, Liu J, Rozeboom AM, Harris BT, Blommaert E, Gomez A, Garcia RE, Zhou Y,…
Dr. Panigrahi co-authors a Nature Communications proteogenomic study of neoantigens
January 3rd, 2022
Proteogenomic discovery of neoantigens facilitates personalized multi-antigen targeted T cell immunotherapy for brain tumors. Rivero-Hinojosa S, Grant M, Panigrahi A, Zhang H, Caisova V, Bollard CM,…
More evidence that glycosylkation affects SARS-CoV-2 infectivity
January 3rd, 2022
SARS-Cov-2 Spike binding to ACE2 is stronger and longer ranged due to glycan interaction. Huang Y, Harris BS, Minami SA, Jung S, Shah PS, Nandi S, McDonald KA, Faller R Biophysical Journal (2022)…
Non-redundant function of 6-O-endosulfatases in the developing spinal cord
December 9th, 2021
Sulf2a controls Shh-dependent neural fate specification in the developing spinal cord. Danesin C, Darche-Gabinaud R, Escalas N, Bouguetoch V, Cochard P, Al Oustah A, Ohayon D, Glise B, Soula C Sci…
Inhibition of 6-O-endosulfatases accelerates oligodendrocyte recruitment and remyelination
December 9th, 2021
Overcoming the inhibitory microenvironment surrounding oligodendrocyte progenitor cells following experimental demyelination. Saraswat D, Shayya HJ, Polanco JJ, Tripathi A, Welliver RR, Pol SU,…
O-glycosylation regulates furin cleavage of the SARS-CoV-2 spike
December 9th, 2021
Furin cleavage of the SARS-CoV-2 spike is modulated by O-glycosylation. Zhang L, Mann M, Syed ZA, Reynolds HM, Tian E, Samara NL, Zeldin DC, Tabak LA, Ten Hagen KG. Proc Natl Acad Sci U S A. 2021…
Targeting evolutionarily-conserved N-glycosylation affects SARS-CoV-2 infectivity
December 9th, 2021
Targeting conserved N-glycosylation blocks SARS-CoV-2 variant infection in vitro. Hsiang-Chi Huang, Yun-Ju Lai, Chun-Che Liao, Wang-Feng Yang, Ke-Bin Huang, I-Jung Lee, Wen-Cheng Chou, Shih-Han…
